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MALDI plates with lectins

MALDI plates with proteolytic enzymes

MS spectra of tryptic glycopeptides after enrichment (top red spectrum) using MALDI chip functionalized with Concanavalin A and without enrichment (bottom flipped black spectra).
A/ MS spectrum of enriched glycopeptides WVPAATEAPISSFEPFPTPTAGAS (pep) and its truncated form WVPAATEAPISSFEPFPTPTAGA (pep*) bearing different combinations of hexoses.
B/ MS spectrum of glycopeptide ELSDIFPDHWFHVGGDEIQPNCFNFSTHVTK bearing high mannose glycans (green circle – mannose, blue square – N-acetylglucosamine).

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