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Enzyme columns

John R. Engen, Ph.D.

My lab has been using AffiPro columns for HDX-MS since they became available.

The AffiPro column portfolio has provided us with a wide range of different protease options with outstanding reproducibility, as well as reliable, long-term performance.

John R. Engen, Ph.D.
Distinguished Professor and James L. Waters Chair in Analytical Chemistry
Dept. of Chemistry & Chemical Biology
Northeastern University

Mike Guttman, Ph.D.

We’ve been using AffiPro protease columns for several years now.

They have been consistently reliable and remarkably durable. With the availability of a robust glycanase column we now also have a simple approach to improve sequence coverage for glycoproteins.

Mike Guttman, Ph.D.
Associate Professor
Dept. of Medicinal Chemistry
University of Washington

Available enzymes

Optimization of protein digestion is a key step in the overall HDX experiment. Spatial resolution and complete protein sequence coverage are the crucial parameters necessary for obtaining useful structural information.

AffiPro offers a set of different types of enzyme columns for on-line digestion with different cleavage preferences.

The selection of enzyme column or their combination together with smart choice of denaturant agents and flow rate boosts digestion performance and allows to achieve full coverage of the protein sequence with many overlapping peptides.



Interested in the AffiPro MALDI chip technology? There will be two lectures at the ASMS 2024 in the Clinical Analysis: Innovations section covering this topic: TOE am 8:50 (Dvorak et al.) and TOE 9:10 (Volny et al.)


Stop by at the AffiPro booth 326 during the upcoming ASMS 2024 conference to see what is new! We are looking forward to meeting you in Anaheim!


The highly anticipated HDX meeting in Monterey is quickly approaching! We can’t wait to see you there in April

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