Home » Enzyme columns

Enzyme columns

John R. Engen, Ph.D.

My lab has been using AffiPro columns for HDX-MS since they became available.

The AffiPro column portfolio has provided us with a wide range of different protease options with outstanding reproducibility, as well as reliable, long-term performance.

John R. Engen, Ph.D.
Distinguished Professor and James L. Waters Chair in Analytical Chemistry
Dept. of Chemistry & Chemical Biology
Northeastern University

Mike Guttman, Ph.D.

We’ve been using AffiPro protease columns for several years now.

They have been consistently reliable and remarkably durable. With the availability of a robust glycanase column we now also have a simple approach to improve sequence coverage for glycoproteins.

Mike Guttman, Ph.D.
Associate Professor
Dept. of Medicinal Chemistry
University of Washington

Available enzymes

Optimization of protein digestion is a key step in the overall HDX experiment. Spatial resolution and complete protein sequence coverage are the crucial parameters necessary for obtaining useful structural information.

AffiPro offers a set of different types of enzyme columns for on-line digestion with different cleavage preferences.

The selection of enzyme column or their combination together with smart choice of denaturant agents and flow rate boosts digestion performance and allows to achieve full coverage of the protein sequence with many overlapping peptides.

News

Jun
2025

AffiPro is launching new RNase
T1 Column
for
online
RNA
Digestion

  • Imobilized RNAase T1 for online digestion compatible with LCMS in a single workflow
  • Proven RNAase T1 specificity with good reproducibility in subsequent LCMS runs
  • Rapid analysis and increased throughput with minimal sample consumption

May
2025

The Institute of Microbiology of the Czech Academy of Sciences is entering AffiPro s.r.o as a minority shareholder and strategic partner. By forming the joint venture with one of the largest institutes of the Czech Academy of Sciences, AffiPro is becoming its official spin-off company, and gains access to shared resources and priority licensing of technologies. Institute of Microbiology now owns 13.75% of the new entity, while 86.25% remains in the possession of the current owners of AffiPro. This join effort aims at Institute of Microbiology plans to commercialize some of its unique technologies while also helps AffiPro to intensify its activities in development of new tools for biotech industry and structural mass spectrometry.
https://lnkd.in/eAq6gMJE

Apr
2025

Join Us at ASMS 2025 in Baltimore
📍 Booth #311 | 📅 June 1–5, 2025 | 🏙 Baltimore Convention Center

Visit us at Booth 311 to discover the latest in structural mass spectrometry and innovative solutions from our team.

Whether you want to learn about our newest developments, explore potential collaborations, or just catch up, we’d love to connect.

All news