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Enzyme columns

John R. Engen, Ph.D.

My lab has been using AffiPro columns for HDX-MS since they became available.

The AffiPro column portfolio has provided us with a wide range of different protease options with outstanding reproducibility, as well as reliable, long-term performance.

John R. Engen, Ph.D.
Distinguished Professor and James L. Waters Chair in Analytical Chemistry
Dept. of Chemistry & Chemical Biology
Northeastern University

Mike Guttman, Ph.D.

We’ve been using AffiPro protease columns for several years now.

They have been consistently reliable and remarkably durable. With the availability of a robust glycanase column we now also have a simple approach to improve sequence coverage for glycoproteins.

Mike Guttman, Ph.D.
Associate Professor
Dept. of Medicinal Chemistry
University of Washington

Available enzymes

Optimization of protein digestion is a key step in the overall HDX experiment. Spatial resolution and complete protein sequence coverage are the crucial parameters necessary for obtaining useful structural information.

AffiPro offers a set of different types of enzyme columns for on-line digestion with different cleavage preferences.

The selection of enzyme column or their combination together with smart choice of denaturant agents and flow rate boosts digestion performance and allows to achieve full coverage of the protein sequence with many overlapping peptides.

News

Feb
2026

AffiPro Joins HDX-MS 2026 as Silver Sponsor

We are thrilled to announce that AffiPro is a Silver Sponsor of the 5th International Conference on Hydrogen Deuterium Exchange Mass Spectrometry, taking place in Strasbourg, 9–13 March 2026.

Aug
2025

AffiPro Sponsors the Clinical & Translational Omics Symposium 2025

AffiPro is proud to be a sponsor of the Clinical & Translational Omics Symposium, taking place October 30 – November 2, 2025.

The symposium will bring together leading researchers, clinicians, and industry representatives to explore the latest advances in omics technologies and their impact on clinical and translational research.

Jun
2025

AffiPro is launching new RNase
T1 Column
for
online
RNA
Digestion

  • Imobilized RNAase T1 for online digestion compatible with LCMS in a single workflow
  • Proven RNAase T1 specificity with good reproducibility in subsequent LCMS runs
  • Rapid analysis and increased throughput with minimal sample consumption
All news